Assignment Task:

 

INTRODUCTION

  •  The first three laboratory periods involve working with live fruit flies. Scientifically, it is called Drosophila melanogaster.
  •  Drosophila melanogaster (dros “fruit”, phila “to like”, melano “brown”, gaster “belly”) is the most studied multicellular organism in regard to Mendelian Genetics.
  •  All of its genes on its 8 chromosomes have been mapped long before the DNA techniques were available.
  •  The haploid number of chromosomes is 4, with one of these being a sex chromosome (X or Y). The males have XY and females XX, so they show sex-linked traits in the same fashion as in humans.

 

TASKS:

 •Practice, observe and learn monohybrid cross in fruit flies
 •Become familiar with the life history of Drosophila melanogaster
 •Learn how to properly handle and culture living organisms
 •Recognize morphological features of the fruit flies
 •Determine how several mutations of the fruit flies are inherited
 •Find out how many genes are responsible for each mutant phenotype
 •Explore whether the alleles of the mutant phenotypes is dominant, recessive, co-dominant or semi dominant
 •Determine if the genes are assorting independently or dependently (i.e. genetically-linked)
 •Use chi-square statistics to test and evaluate experimental results

 

Parts of the experiments:

  • PART 1: Life Cycle of Drosophila melanogaster
  • PART 2: Culturing and Handling Drosophila melanogaster
  • PART 3: Parental Crosses of Drosophila melanogaster
  • PART 4: (Open Lab 1) Count F1 Flies and Establishing F1 X F1 Crosses
  • PART 5: (Open Lab 2) Counting F2 Flies and Writing Formal Lab Reports

 

 

life cycle

 

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  • Uploaded By : Pearl
  • Posted on : October 29th, 2018

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